Brain cancer is a deadly disease with a treatment regime that remains unchanged for decades. In brain cancer biology, intra-tumoural heterogeneity is fast becoming an essential element to understand the complex nature of solid tumours. With the advent of single cell sequencing, tumours that appear homogenous by pathology are revealing unforeseen heterogeneity in the tumour sample. Indeed, in high-grade glioma (i.e., glioblastoma, GBM), increased ITH is associated with more aggressive disease. The next step in investigating ITH is to spatially discern these clusters of transcriptionally distinct cell types to identify specific niches in which they reside and cells they associated with. Here, we aimed to investigate the tumour and immune heterogeneity of high-grade glioma through spatial transcriptomics. We have obtained surgical samples from Anaplastic Astrocytoma (AA, IDH1-mutant) and Glioblastoma (GBM, IDH1-wt) patients. We evaluated spatial regions of interest with increased cycling cells (Ki67 staining) and immune infiltrate (CD45 staining) by NanoString GeoMx whole transcriptome analysis. Individual copy-number alterations and transcriptional profiles suggest that there are deeper levels of heterogeneity in these samples than initially appreciated, likely driven by cycling cells and involving cell-to-cell communication with spatial significance. This primary study reveals high levels of intra-tumoural heterogeneity in high-grade glioma, and a diverse immune landscape between glioma subtypes, likely impacting response to immunotherapy treatments.