The drive towards optimized spatial biology tools requires a balance of high resolution, broad dynamic range, rapid throughput, robust chemistry, and advanced analytics. The ChipCytometryTM platform is an open-sourced approach to spatial biology that is run on the newly launched CellScapeTM instrument, which comprises a highly optimized imaging system that integrates precision imaging with a fully automated and high-throughput workflow. The true range of biological expression intensities exceeds the dynamic range of even the most advanced optical sensors, meaning that traditional fluorescence microscopy approaches may truncate expression intensities and inaccurately quantify key expression values. To address this issue, the CellScape employs a novel multi-exposure high dynamic range (HDR) image acquisition pipeline that enables detection and quantification of both high- and low-expressing targets simultaneously. The ChipCytometry platform also relies on standard fluorescently labelled antibodies which can be sourced from any commercial vendor to unlock a broad range of highly specialized applications.