T-cells are a critical component of the adaptive immune system and play a key role in immunological surveillance. Upon engagement of T-cell receptor (TCR), CD4+ and CD8+ T-cells acquire effector functions through a complex interplay between mRNA and proteins yet to be fully understood. To investigate the temporal landscape of activated T-cells, we conducted an integrative proteomic and transcriptomic analysis of primary human CD4+ and CD8+ T-cells at multiple time points following in vitro activation with anti-CD3/CD28 beads. Our data reveal a time-dependent dissociation between the transcriptome and proteome contents of T-cells following activation: the onset of activation was driven by rapid changes of the mRNA content with sluggish increase in protein synthesis, ultimately leading to rewired transcriptome and proteome. We surprisingly found that CD4+ and CD8+ T-cells became transcriptionally more divergent while their proteome became more similar over the time course of activation. Several changes in the content of mRNAs and proteins associated with metabolic pathways were detected through KEGG pathway analysis in both subsets of T-cells. Following TCR-engagement, a rapid upregulation of the glutamine transporters SLC1A5, SLC7A5 and SLC3A2 paralleled by downregulation of the glucose transporter 1 (GLUT1) correlated with increase of CD69 and decrease of CD226 expression on T-cells, markers of early phase of activation. Prolonged activation restored cell surface levels of GLUT1 and increased enzymes involved in glucose metabolism, resulting in T-cell proliferation. Our data provides a comprehensive framework for understanding the main temporal changes that regulate metabolic pathways governing the acquisition of effector functions by CD4+ and CD8+ T-cells.